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KMID : 0380620110430040446
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Korean Journal of Food Science and Technology
2011 Volume.43 No. 4 p.446 ~ p.452
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Biochemical and Molecular Identification of Antibacterial Lactic Acid Bacteria Isolated from Kimchi
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Kim Soo-Young
Kim Soo-Young
Son Ji-Soo
Lee Si-Kyung
Park Kab-Joo
Park Myeong-Soo
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Abstract
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Total 480 lactic acid-producing bacteria were isolated from five kinds of kimchi, and their antibacterial activity was tested against Salmonella enterica serovar Typhimurium, Bacillus subtilis, and Pseudomonas aeruginosa using an agar diffusion assay. Among them, 340 isolates showed a halo on MRS agar against one or more indicator strains, which were identified using multiplex PCR, an API 50CHL kit, and a 16S rDNA sequence analysis. As a result, 169 Lactobacillus plantarum, 20 Lactobacillus fermentum, two Lactobacillus paracasei ssp. paracasei, two Lactobacillus sp., and 15 Pediococcus sp. were identified. This may be the first report on the isolation of antibacterial Lactobacillus fermentum from kimchi.
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KEYWORD
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antibacterial activity, kimchi, lactic acid bacteria, identification
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